Critical Appraisal

The impact of four harvesting techniques on the cell viability and osteogenic behaviour of cells in autogenous bone grafts: A critical appraisal of an experimental study

Andre W. van Zyl, Johan Hartshorne, Alonso Carrasco-Labra
Open Journal of Implant Dentistry | Vol 1, No 1 | a6 | DOI: https://doi.org/10.4102/ojid.v1i1.6 | © 2013 Andre W. van Zyl, Johan Hartshorne, Alonso Carrasco-Labra | This work is licensed under CC Attribution 4.0
Submitted: 30 January 2013 | Published: 26 March 2013

About the author(s)

Andre W. van Zyl, Department of Periodontics and Oral Medicine, University of Pretoria, South Africa
Johan Hartshorne, Visiting Professor: Department of Periodontics and Oral Medicine, School of Dentistry, University of Pretoria, South Africa
Alonso Carrasco-Labra, Department of Oral and Maxillofacial Surgery and Evidence-Based Dentistry Unit, Faculty of Dentistry, Universidad de Chile, Chile


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Abstract

The investigators tested the null hypothesis that there would be no differences between the different bone harvesting techniques with regard to cell viability, cell activity and osteogenic potential of grafted cells. Bone grafts were harvested from the mandibles of 12 miniature pigs using four different harvesting techniques: bone milling, bone scraping, bone drilling (bone slurry) and piezosurgery. Cell viability was determined according to an immunoassay of released signalling molecules and gene expression that affect bone formation and resorption. The osteogenic activity of conditioned graft-sampled media was assessed in a bioassay using isolated bone cells. Cells in autogenous bone grafts obtained by using a bone mill and a bone scraper showed a higher viability and a stronger osteogenic potential than those from piezosurgery and bone drilling (slurry). This study contributed towards the understanding of the impact of harvesting techniques on the viability and osteogenic behaviour of grafted cells.


Keywords

autogenous bone grafts; bone drilling; bone harvesting; bone milling; bone scraping

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